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. 2019 Nov 1;9:15803. doi: 10.1038/s41598-019-52225-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Restored expression of downregulated miRNAs reduce the ability of CRC cell lines to migrate and invade. CRC cell lines (CT26, SW620, HCT116) transfected with miR-378f, miR-605, miR-1976, or negative control (NC) mimics were plated into migration (a–c), and invasion (d–f) assays. Migration and invasion data presented as average number of cells in 5 fields + standard deviation with representative images of each below the graphs. Larger purple stained cells in the representative images signify migrated or invaded cells. Statistics generated using student t-test; N = 3, *p < 0.05, **p < 0.005.