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. 2019 Nov 1;9:15864. doi: 10.1038/s41598-019-52436-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunolabeling of spermatozoa samples from DNAH6-mutant patients and normal controls (NC). Immunostaining of acetylated α-tubulin (green) and DNAH6 (red) in spermatozoa of NC and patients (P1 and P3). Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue). DNAH6 is specifically expressed in the sperm tail. Sperm tails in patients were short compared to those in the NC. Scale bars = 5 μm.