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. 2019 Nov 1;9:15847. doi: 10.1038/s41598-019-52127-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Modulation of RGD-endothelial interactions by microparticle-carried ligands. (a) Representative images show localization of cRGD-MPs (white arrowheads - upper panel; shown in green with arrowheads - lower panel) and CD-31 staining (red - lower panel) on TNF-activated endothelial cells (blue - DAPI). (b) Bindings of cRGD-MPs (0.1 µg/ml) in isolated, pressurized MCA, before and after incubation with TNF. (c) Representative images show VTN-MP bindings in TNF-exposed MCA with prior intraluminal delivery of cRGD peptide (100 μg/ml) or cRGD-MPs (0.1 µg/ml). (d) Summary data of VTN-MP bindings in TNF-exposed MCA with cRGD peptide or cRGD-MPs. IEL: Internal Elastic Lamina. Scale bar: 10 μm. Data are mean ± SEM; *p < 0.05.