Fig. 1.

Isotope-labeling schemes and ¹³C solid-state NMR spectra of hBD-3 analog. a The ¹³C, ¹⁵N-labeled residues are in red for VALIG peptide, and in blue for IVLG peptide in both the amino acid sequence and solution monomeric structure of wt-hBD-3 (PDB 1KJ6). b 1D ¹³C spectra of VALIG peptide in POPC/POPG bilayers at 269 K and 298 K. From top to the bottom are INEPT, DP, and CP spectra measured at 298 K, and CP spectrum at 269 K. Dashlines indicate the resolved peptide peaks. c 2D ¹³C-¹³C correlation spectra of VALIG with 100-ms DARR mixing. The rigid and mobile components are selected using ¹H–¹³C CP and ¹³C DP, respectively. Greek letters indicate carbon sites and superscripts annotate conformers. I30^aαβ: carbon α to carbon β cross peak of subtype-a I30