Fig. 3.

Site-specific depth of insertion of hBD-3 analog in POPC/POPG bilayers. a 2D ¹³C–¹H correlation spectrum of VALIG, with 225-ms ¹H spin diffusion and the representative ¹H cross-sections of L21^aCα at different ¹H mixing times. The water-to-peptide (purple) and lipid-to-peptide (black) ¹H polarization transfer curves of b, well-inserted residues and c, surface-bound residues are shown. The carbon site and the best-fit distances are labeled for each panel. Error bars are standard deviations that are propagated from signal-to-noise ratios. d I30 is far from both water and lipids. e Summary of distances from water and lipid acyl chains to the peptide