Fig. 2.

PPAA outperforms commercially available peptide delivery reagents. Comparison of peptide delivery reagent-mediated a uptake (numbers above the bars denote the fold increase in peptide uptake compared with the peptide alone) and b retention of the YARA-MK2i peptide over time following treatment removal. c Representative colocalization images and d quantification of peptide colocalization with the endosomal dye LysoTracker Red demonstrating PPAA-mediated endosomal escape and intracellular YARA-MK2i peptide delivery; *p < 0.05 vs. all other treatment groups. e Evaluation of delivery reagent-mediated cytotoxicity compared with delivery of the YARA-MK2i peptide alone (10 µM peptide); *p < 0.05 vs. treatment with the peptide alone; statistical analyses—one-way ANOVA followed by Tukey’s post hoc test. Data are presented as means ± SEM graphically