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. 2019 Nov 1;10:5011. doi: 10.1038/s41467-019-13028-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — FASN is essential for PyMT-dependent transformation in vitro. a Representative images of the soft agar colony assay and quantitation of colonies per well recovered from each of the four genotypes (n = 6 biological replicates for each genotype). Scale bars, 500 μM. b FASN mRNA and protein levels detected in soft agar colonies recovered from the FASN^lox/lox-PyMT and FASN^∆/∆-PyMT MEFs, respectively. c Cell replication rates after 1, 2, or 3 days for each genotype (n = 3). Presented data are the mean values ± standard deviation (SD). ***P < 0.001; n.s., not statistically significant; Student’s t test. d Flow cytometry analysis of apoptosis. Percentages of cells in apoptosis are represented (FASN^lox/lox-Empty, n = 7; FASN^∆/∆-Empty, n = 8; FASN^lox/lox-PyMT, n = 7; FASN^∆/∆-PyMT, n = 5). Presented data are the mean values ± standard deviation (SD)