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. 2019 Oct 16;116(44):22282–22287. doi: 10.1073/pnas.1816521116

Fig. 4.

Fig. 4.

β-AR-dependent expression of α- and β-globin genes. (A) H3S28p-enriched genomic regions within the Hbb-bt/bs and Hba-a1 locus in comparison with all differentially expressed genes in WT after Iso (average). These genes correspond to 1,188 genomic regions. The H3S28p enrichment peak locates to the TSS (S) of Hbb-bt/bs and to the transcriptional end site (E) of Hba-a1. (B) Relative mRNA expression of adult hemoglobin genes (Hbb-bt/bs, Hba-a1/a2) in AMVMs from WT or DKO under basal conditions and after Iso treatment (100 nM, 24 h). Colors indicate different hearts; n.s., not significant; *P < 0.05, ****P < 0.0001. (C) Relative expression levels of Hbb-bt/b1 and Hba-a1/a2 in heart tissue from WT and CaMKII-TG mice treated with metoprolol (ME) or H2O detected with qPCR; n.s., not significant; *P < 0.05. (D and E) Immunoblots and quantification showing CaMKII, H3S28p, H3, and α- and β-globin levels of Iso-treated (100 nM, 24 h) AMVMs from WT and DKO mice. Colors indicate different hearts; n.s. not significant; *P < 0.05; **P < 0.01; ***P < 0.001.