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. 2019 Oct 14;116(44):22322–22330. doi: 10.1073/pnas.1909666116

Fig. 4.

Fig. 4.

Exposure to pathogens drives a rapid and SKN-1–dependent loss of somatic lipids. (A) Somatic lipid depletion requires live bacteria. (A) Bactericidal treatment of OP50 bacteria with UV+antibiotics (“killed”) or FUdR reduces Asdf in skn-1gf animals relative to mock-treated bacteria. (B and C) Constitutive activation of the p38 MAPK pathway in nsy-1gf(ums8) mutant animals displays age-dependent somatic depletion of fat (Asdf) at 144 h postfeeding; representative image (B) and quantification of population (C). (D) Lipid reallocation in nsy-1gf(ums8) mutant animals requires skn-1. (E and F) Exposure of 72-h postfed animals to P. aeruginosa results in the rapid loss of somatic lipid stores; representative image (E) and quantification of population (F). All experiments were performed in a minimum of biological triplicate and lipid distribution was assessed in at least 200 animals. (Scale bar, 50 μm.) ****P < 0.0001.