Fig. 5.

Redirection of SKN-1gf negates pathogen resistance. (A–B) Pathogen sensitivity assays. Wild-type (black), skn-1gf(lax188) mutants (red), wdr-5lf(ok1417) mutants (blue), or wdr-5lf(ok1417);skn-1gf(lax188) double mutants (green) were exposed to P. aeruginosa (A) “fast-kill” or (B) “slow kill” pathogen stress plates. Activated SKN-1 supports resistance to pathogenic P. aeruginosa specifically on fast-kill plates, which is abolished in the absence of H3K4me3. (C) Model for the impact of SKN-1gf on physiological responses to P. aeruginosa secreted factors “fast killing.” “?”, the role of SKN-1gf on colonization “slow killing” is unclear and requires further study. All experiments were performed in a minimum of biological triplicate.