Fig. 6.

SKN-1 activation drives lipid utilization during Asdf. (A–C) Analysis of the mRNA reads of the indicated genes related to lipid utilization: (A) lipid beta-oxidation and (B) lipolysis; and (C) lipid binding, which collectively reveal a scenario where activated SKN-1 drives lipid utilization while suppressing de novo synthesis (see also SI Appendix, Fig. S10). (D) Fixed Nile Red staining reveals skn-1gf(lax188) mutants have a ∼60% reduction in total fat (n = 30). (E–H) GCMS analysis of total fatty acids in the triglyceride fraction of wild-type (black) and skn-1gf(lax188) mutant (red) animals fed an OP50 diet at day 2 of adulthood when Asdf is most prominent, 2-tailed t test. See also Datasets S1–S4 for all RNA-seq measurements. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. FPKM, fragments per kilobase of transcript per million mapped reads.