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. 2019 Oct 15;116(44):22205–22211. doi: 10.1073/pnas.1909227116

Fig. 5.

Fig. 5.

Myosin II motor variants produce slowed cell intercalation. (A) Schematic of cell intercalation. (B) Stills from movies showing cell rearrangements, with time indicated relative to vertex formation. (Scale bar: 5 μm.) (C) AP edge contraction rates were decreased in MyoII-R707C, MyoII-N98K, and MyoII RNAi embryos compared with MyoII-WT. *P < 0.05, t test. Edge contraction was slower in MyoII-N98K than MyoII RNAi (P = 0.05) (t test). (D) The percentage of AP edges that completed contraction was decreased in MyoII-R707C, MyoII-N98K, and MyoII RNAi embryos compared with MyoII-WT. *P < 0.05, χ2 test. (E) Vertex resolution rates were not significantly different in all genotypes. P > 0.1, t test (τ = time for a vertex to resolve to form a new edge >1 μm in length; 1/t = vertex resolution rate). (F) The percentage of vertices that resolved directionally to form DV edges was decreased in MyoII-R707C compared with MyoII-WT. *P < 0.05, χ2 test. In C and E, the boxes indicate the 25th to 75th percentiles, with a line at the median, and the whiskers indicate the 10th to 90th percentiles. Mean, plus sign. In D and F, data are mean ± SEM between embryos. In C and D, there are 3 embryos per genotype and 20 edges per embryo; in E and F, there are 4 to 5 embryos per genotype and 15 to 24 vertices per embryo.