Fig. 3.

SghR controls the transcriptional expression of sghA. (A) RT-PCR analysis of the sghA expression patterns in wild-type strain A6 and its sghR mutant at different time intervals postinoculation in BM medium with mannitol as the sole carbon source. (B) Electrophoretic mobility-shift assay (EMSA) analysis of SghR binding to the sghA promoter. The unrelated transcriptional regulator AttJ from A. tumefaciens and the promoter Ppa0305 from P. aeruginosa were used as specificity controls. Purified proteins of SghR and AttJ were mixed with the biotin-labeled probes (1 nM) at various concentrations, and reaction mixtures were separated by electrophoresis. Experiments were repeated at least 3 times with similar results, and 1 set of representative data is presented.