Fig. 3.

ITC binding isotherm for binding of CinA^wPip to CinB^wPip yielded a K_d of 25 ± 1.3 nM. This tight interaction provides support to the hypothesis that the cognate pair exists as a toxin–antidote system. (Top) Raw injection data over time. DP, differential power. (Bottom) Integrated heats over the course of the reaction. Error bars provided by NITPIC during integration of the raw thermogram represent heat changes for each injection. n = 3.