Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 15;116(44):22314–22321. doi: 10.1073/pnas.1914571116

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Published under the PNAS license.

PMC Copyright notice

Fig. 5. — Expression of the cinA–cinB^wPip genes in flies induces CI-like embryo killing and rescue phenotypes. (A and B) Two strategies were used to generate transgenic flies. cinA^wPip was inserted into the second chromosome and cinB^wPip was inserted into the third chromosome (UAS:cinA/UAS:cinA; UAS:cinB/UAS:cinB) (A), or cinA^wPip and cinB^wPip were linked by a T2A viral sequence (yellow) and inserted into the third chromosome (UAS:cinA-T2A-cinB/UAS:cinA-T2A-cinB) (B). (C) Crosses with flies transgenic for either cinA^wPip or cinB^wPip alone expressed using the NGT driver (highlighted in orange). Data in burgundy represent CI-inducing crosses while green represents either rescue or weakening of CI induction by the CinB-K636A mutation. All control crosses are shown in gray. n = 40 to 64. (D) Crosses with flies transgenic for the entire cinA–cinB^wPip operon under control of the NGT driver. n = 38 to 58. Error bars in C and D represent SD of the mean; **P < 0.01, ***P < 0.001, ****P < 0.0001 by ANOVA with multiple comparison between all groups. (E) Transgenic CI in crosses with flies transgenic for the entire cinA–cinB^wPip operon expressed using the strong maternal triple driver (highlighted in red) could be rescued by transgenic cinA^wPip females. Vertical lines represent medians. n = 23 to 57. ****P < 0.0001 by 2-tailed Mann–Whitney U test.