Fig. 2.

NKT2 cells require TCR signals for steady-state production of IL-4. (A) Representative histogram of Nur77^GFP expression on subsets of thymic iNKT cells from BALB/c Nur77^GFP or BALB/c Wt mice. Data are representative of 2 experiments. n = 10. (B) Normalized Nur77^GFP geometric mean fluorescence intensity (gMFI) (to DP) of thymic stage 0 iNKT, CCR7⁺ iNKT precursor (NKTp), NKT1, NKT17, and IL-4⁺ NKT2 cells from BALB/c Nur77^GFP mice. Data are pooled from 2 experiments. n = 10. Ordinary 1-way ANOVA, ****P < 0.0001. (C) Experimental scheme showing iNKT-enriched thymocytes from CD45.1⁺/CD45.2⁺ BALB/c KN2 mice intrathymically transferred into BALB/c Wt (CD45.2⁺/CD45.2⁺ or CD45.1⁺/CD45.1⁺) or BALB/c Cd1d KO (CD45.2⁺/CD45.2⁺) mice; 9 d later transferred donor iNKT cells were isolated by magnetic enrichment. (D) IL-4 (huCD2) and PLZF in donor iNKT cells 9 d after intrathymic transfer into BALB/c Wt or BALB/c Cd1d KO host mice. Data are representative of 5 experiments. n = 16 (E) Percentage and cell number of huCD2⁺ cells in transferred donor iNKT cells 9 d after intrathymic transfer. Data are pooled from 5 experiments. n = 16. Unpaired t test, ***P = 0.0002, ****P < 0.0001.