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. 2019 Oct 14;116(44):22189–22195. doi: 10.1073/pnas.1906484116

Fig. 6.

Fig. 6.

FOXB2 induces Wnt activity and neuroendocrine differentiation in prostate cancer cells. (A) TOPflash assay in PC-3 cells. FOXB2, but not FOXB1, synergized with Wnt3a/R-spondin 3 (W/R) in pathway activation. (B) TOPflash assay in PC-3 cells following depletion of FOXB2 with 2 independent siRNAs. Scrambled siRNA was used as control. (C) qPCR expression analysis of ENO2 (encoding neuron-specific enolase; NSE), AXIN2, and WNT7B in PC-3 cells following depletion of FOXB2. (D) Expression analysis of androgen receptor (AR) and neuroendocrine differentiation markers ASCL1 and ENO2 in LNCaP cells. FOXB2 was over-expressed transiently, and data were normalized to empty vector control. (E) Immunofluorescence staining of NSE and FOXB2 in stably transfected LNCaP cells following G418 selection for more than 2 wk. FOXB2-overexpressing cells exhibited high levels of NSE protein. Nuclei are shown in blue (*P < 0.05, **P < 0.01, and ***P < 0.001 versus control).