Fig. 8.

Model of DdMyo7 Inhibition and activation states of DdMyo7. The major domains are shown as in Fig. 1. (Left) The inactive state where MF2 interacts with the motor domain to autoinhibit activity. (Middle) Active DdMyo7 that is dimerized via partner binding and dimerization mediated by a dimerization region (gray rectangle) of weak affinity. (Right) Proposed active state for the ΔSAH-SH3 mutant that lacks the central region of the DdMyo7 encompassing the SAH, PLA, MF1, and SH3 domains. Shown here is a pair of myosin heavy chains dimerized by partner binding to the MF2 and extended motor domains that can bind 2 actin filaments.