Table 1. Characteristics of designed primers and related genes used in this study .
| Targeted genes | Sequence (5′ 3′) | Tm (°C) |
Amplicon size (bp) |
Reference |
| 16S rRNA | F GCTCAACCAGGCACAAAAACA R TCCACGCCTAGTTGGGTCTA |
59 | 300 | Present study |
| Vaginolysin (vly) | F GCACCAGATAGCCCAGCAGA R TTCGGTGCCGTACTCATCCC |
62 | 540 | Present study |
| Phospholipase C (pho) | F GCGTGCTCCGCTTCGATTAG R TCCGCGGTAACGCTTCTCTT |
61 | 421 | Present study |
| Sialidase (sld) | F AGCCCGCATATCCCGTATCG R GGACCTGGCCAACATGGAGT |
62 | 454 | Present study |