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. Author manuscript; available in PMC: 2020 Jan 30.
Published in final edited form as: Cell Rep. 2019 Jul 30;28(5):1127–1135.e4. doi: 10.1016/j.celrep.2019.06.087

Figure 1. Local APC Targeting with Anti-CD40Ab following Subcutaneous Administration.

Figure 1.

(A) Rhesus macaques were immunized subcutaneously or i.v. with Alexa-Fluor-680-labeled anti-CD40Ab and poly(IC:LC). For s.c. immunization, indicated samples were collected at 24 and 48 h. Shown are representative histograms of the monocyte Alexa Fluor 680 signal from immunized (gray) or unimmunized (black) animals.

(B) Proportion of the CD40 Alexa Fluor 680 signal (mean fluorescent intensity, MFI) of APCs in collected tissues.

(C–F) CD40 Alexa Fluor 680 MFI on APCs.

(G) Monocyte binding following s.c. or i.v. administration. Each data point represents a separate animal (n = 3, mean ± SEM). See also Figure S1.