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. Author manuscript; available in PMC: 2020 Jun 27.
Published in final edited form as: J Med Chem. 2019 Jun 12;62(12):5810–5831. doi: 10.1021/acs.jmedchem.9b00136

Figure 8. Antiviral effect of compounds 1, 8g and 21b correlate with functional inhibition of AAK1.

Figure 8.

Dose response of AP2M1 phosphorylation to treatment with 1 (A), 8g (B), and 21b (C) by Western analysis in lysates derived from Huh7 cells. Representative membranes (from two independent experiments) blotted with anti-phospho-AP2M1 (pAP2M1), anti-AP2M1 (AP2M1), and anti-Actin (Actin) antibodies and quantified data of pAP2M1/actin protein ratio normalized to DMSO controls are shown. ** p < 0.01, *** p < 0.001 by 2-tailed unpaired t test.