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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Cancer Res. 2019 Jul 16;79(21):5482–5489. doi: 10.1158/0008-5472.CAN-19-1302

Figure 2. ARID1A transcriptionally represses Cd274.

Figure 2.

(A) Expression of Cd274 mRNA and PD-L1 protein in ARID1A wildtype control and ARID1A knockout mouse ovarian ID8-Defb29/Vegf cells treated with or without 20 ng/ml IFNγ determined by qRT-PCR (left) or immunoblot (middle). The cell surface expression of PD-L1 in these cells was determined by flow cytometry analysis (right). (B) The indicated ID8-Defb29/Vegf cells treated with or without 20 ng/ml IFNγ cells were subjected to ChIP analysis for the Cd274 gene promoter using the indicated antibodies or an isotype-matched IgG control. Error bars represent ± S.E.M. n= 3 independent experiments.