Figure 2.

Anticarcinogenic effects of NR4A1 inactivation are due to induction of IL-24. Rh30 cells were transfected with siNR4A1 or treated with 20 mM CDIM8 in the absence or presence of siIL-24; effects on protein expression (A) cell proliferation (B), induction of Annexin V staining (C), and cell migration (D) were determined as outlined in the Materials and Methods. Results are expressed as means ± SD for at three replicate determinations for each treatment group (B, C + D) and significant (p<0.05) effects (*) and reversal of these responses (**) are indicated.