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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Cancer Immunol Res. 2019 Aug 28;7(11):1837–1848. doi: 10.1158/2326-6066.CIR-19-0229

Figure 7. STING is essential for agonist-induced enhanced antigenicity in melanoma cells.

Figure 7.

(A) WM39, sh-control and sh-STING cells were cocultured with TIL 195 for 24 h in the presence or absence of 2’3-cGAMP. IFN-γ levels in supernatants were measured using ELISA. Data are presented as mean ± SD of duplicate samples. P-values were calculated by one-way ANOVA (**P < 0.01, ***P < 0.001, ****P < 0.0001, ns = not significant). (B) 51Cr cytotoxicity assay using WM39, sh-control and sh-STING cells as target cells and TIL 195 as effector cells at the indicated effector/target (E/T) ratios with or without 2’3’-cGAMP. Data represent the mean ± SD of quadruplicate wells. (C) Lytic activity of TIL 195 against indicated targets with or without 2’3’-cGAMP stimulation was measured in three independent experiments, one of which is shown.