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. 2019;20(3):687–692. doi: 10.31557/APJCP.2019.20.3.687

Table 1.

Primers Used for PCR with Thermal Cycling Conditions

Primer Sequence PCR thermal cycling conditions
First Cycle EBNA 3C
5′-GAGAAGGGG AGC GTG TGT TGT-3′
5′ -GCT CGT TTT TGA CGT CGG C -3′
95°C for 10 min,
94°C for 45 s, 54.5°C for 45 s,
72°C for 45 s: 35 cycles 72°C for 10 min; final extension
Nested Cycle EBNA 3C
′-TCA TAG AGG TGA TTG ATG TT-3′
5′-ATG TTT CCG ATG TGG CTT AT-3′
95°C for 10 min,
94°C for 45 s, 54.5°C for 45 s,
72°C for 45 s: 35 cycles 72°C for 10 min; final extension