Table 1.
Primer | Sequence | PCR thermal cycling conditions |
---|---|---|
First Cycle | EBNA 3C 5′-GAGAAGGGG AGC GTG TGT TGT-3′ 5′ -GCT CGT TTT TGA CGT CGG C -3′ |
95°C for 10 min, 94°C for 45 s, 54.5°C for 45 s, 72°C for 45 s: 35 cycles 72°C for 10 min; final extension |
Nested Cycle | EBNA 3C ′-TCA TAG AGG TGA TTG ATG TT-3′ 5′-ATG TTT CCG ATG TGG CTT AT-3′ |
95°C for 10 min, 94°C for 45 s, 54.5°C for 45 s, 72°C for 45 s: 35 cycles 72°C for 10 min; final extension |