Table 1.

Name of Primers, Primer Sequence, and Annealing Temperature Used in PCR Amplification of NUDT15 Gene

Primer	Primer sequence 5’-3’	Annealing temperature (°C)	Region	Size (bp)
NUDT15 1st- F	CAA AGC ACA ACT GTA AGC GAC T	57	exon 1	600
NUDT15 1st - R	GAA AGA CCC AGC TAG CAA AGA C
NUDT15 2nd- F	CGG CCT TCC AAA AGA TTA CA	56	exon 2	580
NUDT15 2nd- R	TGA TCT AAT CAC CTC CCA AGG
NUDT15 3rd- F	AAG CAA ATG CAA AGC ATC AC	57	exon 3	480
NUDT15 3rd- R	GGC TGA AAG AGT GGG GGA TA

F, forward primer; R, reverse primer; bp, base pair