Table 2.

Structural and functional impact of experimental known Bax mutations

Complexes	Bax mutations	Experimental Impact of Bax mutants with Bcl‐2 wild‐type	Pubmed ID	OncoKB	3D Hotspot	Mutation Assessor (impact and score)	SIFT (impact and score)	PolyPhen‐2 (impact and score)
1‐Bcl‐2	Wild‐type	Bind with Bcl‐2	22155216	—	—	—	—	—
2‐Bcl‐2	M74D	Strongly reduced interaction with Bcl‐2	21199865	NA	No	Undefined	Deleterious; 0.01	Benign; 0.091
3‐Bcl‐2	M74E	Strongly reduced interaction with Bcl‐2	21199865	NA	No	Undefined	Deleterious; 0.02	Benign; 0.004
4‐Bcl‐2	G67R	Loss of heterodimerization with Bcl‐2	7475270; 9531611	NA	No	Medium; 2.915	Deleterious; 0.01	Probably Damaging; 0.997
5‐Bcl‐2	E61A	Reduced the affinities with Bcl‐2	21060336	NA	No	Medium; 2.075	Tolerated; 0.73	Possibly Damaging; 0.530
6‐Bcl‐2	R65A	Reduced the affinities with Bcl‐2	21060336	NA	No	Undefined	Tolerated; 0.27	Probably Damaging; 0.972
7‐Bcl‐2	D68A	Greatly reduced the affinities with Bcl‐2	21060336	NA	No	Medium; 2.84	Tolerated; 0.08	Probably Damaging; 0.998
8‐Bcl‐2	E69A	Reduced the affinities with Bcl‐2	21060336	NA	No	Medium; 2.305	Deleterious; 0.00	Possibly Damaging; 0.839
9‐Bcl‐2	R78A	Reduced the affinities with Bcl‐2	21060336	NA	No	Undefined	Tolerated; 0.28	Possibly Damaging; 0.671
10‐Bcl‐2	E61A/ R65A/R78A	Greatly reduced the affinities with Bcl‐2	21060336	NA	No	Medium; 2.075/Undefined/Undefined	Tolerated; 0.73/ Tolerated; 0.27/	Possibly Damaging; 0.530/ Probably Damaging; 0.972/ Possibly Damaging; 0.671
11‐Bcl‐2	I66E/D68R	Completely lost the binding	25703009	NA	No	Undefined/ Undefined	Tolerated; 0.11/ Deleterious; 0.01	Probably Damaging; 1/ Probably Damaging; 1