Figure 5.

Depletion of USP9X results in enhanced cellular sensitivity to DNA‐damaging agents. A and B, MCF‐7 and HeLa cells stably expressing shNC and shUSP9X were treated with or without Olaparib at the indicated concentrations and incubated until colonies were formed. Colonies were stained with crystal violet solution (upper panel). The cloning efficiency (lower panel) was calculated by normalizing each group to vehicle treatment group. (C‐D) MCF‐7 and MDA‐MB‐231 cells stably expressing shNC and shUSP9X #2 were treated with the indicated concentrations of MMS for 24 h. Cells were incubated in fresh medium without MMS for another 24 h, and cell survival was assayed using CCK‐8 kit. In C and D, *P < .05, **P < .01, ***P < .001