Fig. 4.

Heat pre-conditioning suppresses stress granule formation. (A) Representative images showing stress granule formation in midbrain and retina cells of 1 dpf GFP–G3BP1 knock-in zebrafish with indicated treatment paradigms. Control, fish kept at ambient temperature 28°C; 35°C 6 h, fish kept at 35°C for 6 h; 42°C 10 min, fish heat-shocked at 42°C for 10 min; 35°C for 6 h+42°C 10 min, fish first exposed to 35°C for 6 h, then heat-shocked for 10 min at 42°C. Scale bar: 10 µm. (B) Mean±s.e.m. percentage of zebrafish with stress granules detected in the midbrain. Data from two independent experiments, with n=10, 7, 19 and 17 fish for each condition. **P≤0.01 by unpaired Student's t-test.