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. 2019 Oct 21;132(20):jcs226639. doi: 10.1242/jcs.226639

Fig. 1.

Fig. 1.

PEG-4MAL hydrogel crosslinked with GPQ-W peptide does not support tubule formation. (A) Percentage of IMCD cluster area that stained for LIVE (C12-Resazurin) after 14 days of encapsulation in PEG-4MAL hydrogels of different polymer density and crosslinked with GPQ-W peptide (mean±s.e.m.). IMCD multicellular structure (B) projected area and (C) Feret diameter at 14 days after encapsulation in PEG-4MAL hydrogel. (D) Transmitted light images of IMCD cells cultured in PEG-4MAL hydrogels. Scale bar: 100 μm. (E) Percentage of IMCD cluster area that stained for LIVE after 21 days of encapsulation in PEG-4MAL hydrogels (mean±s.e.m.). IMCD multicellular structure (F) projected area and (G) Feret diameter at 21 days after encapsulation in PEG-4MAL hydrogels. (H) Transmitted light images of IMCD cells at 21 days after encapsulation in PEG-4MAL hydrogels. Scale bar: 200 μm. Graph lines represent the mean of the individual data points. Each data point represents one multicellular structure. ****P<0.0001, ***P<0.0002, **P<0.0021 (Kruskal–Wallis with Dunn's multiple comparisons test). Experiments performed with six PEG-4MAL hydrogels per experimental group. Four independent experiments were performed and data are presented for one of the experiments.