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. 2019 Oct 21;132(20):jcs226639. doi: 10.1242/jcs.226639

Fig. 7.

Fig. 7.

Engineered PEG-4MAL hydrogel promotes key features of epithelial tubule differentiation. (A–C) Transmitted light and fluorescence microscopy images of a lumenized IMCD tubule within engineered hydrogel stained for actin (phalloidin) and nuclei (DAPI). White arrows show the lumen. Scale bars: 50 μm. (D) Fluorescence microscopy image of IMCD tubules within engineered hydrogel stained for secreted laminin (LM). Scale bar: 100 μm. (E) Transmitted light and fluorescence microscopy images of IMCD tubules within engineered hydrogel stained for LM, actin (phalloidin) and nuclei (DAPI). Scale bars: 100 μm. Experiments performed with six PEG-4MAL hydrogels per experimental group. Three independent experiments were performed and data are presented for one of the experiments.