Fig. 1.

Identification of pracinostat as a novel attenuator of human lung fibroblast activation. (A) Immunofluorescence images (10× objective magnification) of IMR90 cells treated with indicated doses of TGFβ for 3 days. Scale bars: 100 µm. (B) αSMA intensity with increasing TGFβ dose in IMR90 cells (n=4 biological replicates). (C) Rank ordered plot of 99 small-molecule modulators of epigenetic regulating enzymes. The screen was performed at 2.5 μM in IMR90 cells in technical triplicates. Compounds shown in green are hits which attenuated fibroblast activation. Compounds shown in red are hits that accentuated fibroblast activation. The compound shown in yellow is pracinostat. Toxic compounds are not shown. Dots represent mean values and error bars represent s.e.m.