Fig. 6.

HDAC7 is required for TGFβ-mediated fibroblast activation. (A) qPCR analysis of IPF lung fibroblasts treated with siRNA targeting HDAC7 (siHDAC7) for 72 h then treated with TGFβ for 24 h. Pro-fibrotic genes are shown in red. Putative and confirmed anti-fibrotic genes are shown in light blue. (B) Western blot confirmation of protein knockdown of HDAC7 and its relative effect on acetyl-H3 are shown from a representative blot and quantification from independent replicates. All experiments were performed with cells from three separate IPF lung fibroblast donors. Error bars represent s.e.m. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 (one-way ANOVA with Tukey's multiple comparison test). For HDAC7 knockdown analysis, an unpaired t-test was performed to test for significance.