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. 2019 Oct 22;132(20):jcs232223. doi: 10.1242/jcs.232223

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Tox4 suppression impedes intermediate reprogramming stages. (A–D) The indicated genes were targeted every other day by siRNA transfection of STEMCCA MEFs induced to reprogram. (A) The number of EZH2+ colonies at D9 of reprogramming in S/L+AA. Colony counts were normalized to colony counts in control conditions. Results are shown as the mean±s.d. (n=3 with two biological replicates in total). *P<0.05, **P<0.01, ***P<0.001 (one-way ANOVA with Dunnett's multiple comparisons test compared to control). (B) The number of NANOG+ colonies at D9 of reprogramming in S/L+AA. Colony counts were normalized to colony counts in control conditions. Results are shown as the normalized mean±s.d. of two independent experiments. Results are shown as the mean±s.d. (n=3 with biological duplicates in total). *P<0.05, **P<0.01, ***P<0.001 (one-way ANOVA with Dunnett's multiple comparisons test compared to control). (C) Same as Fig. 2A for KSR+AA. (D) Same as Fig. 2B for KSR+AA. Squares, triangles and circles represent one independent experiment each.