Fig. 2.

Depletion of SMARCAL1 induces DNA damage and checkpoint activation in iSML1 iPSCs. (A,B) The iSML1 iPSCs were cultured for 7 and 14 days in the presence of doxycycline (DOX) to induce SMARCAL1 downregulation and then immunostained. The graphs (top) show quantification of the number of γ-H2AX-positive cells (A) or ATM-pSer1981-positive cells (B). Representative images from triplicate experiments are shown (bottom). (C) Immunoblot detection of the indicated DDR proteins in iSML1 iPSCs after 14 days of continuous treatment with DOX. Lamin B1 was used as the loading control. Data are mean±s.e.m. from three independent experiments. *P≤0.05, **P≤0.01, ***P≤0.001 (two-way ANOVA test). ns, not significant. Scale bars: 10 µm.