Fig. 3.

Enhanced phosphorylation of H2AX and ATM is related to S-phase. (A,B) Analysis of the level of DNA damage in iSML1 iPSCs cells during replication, at either 7 or 14 days. Cells were cultured in doxycycline+ medium as indicated and S-phase cells labelled with EdU for 30 min before sampling. The graphs report the number of γ-H2AX-positive cells (A) or the number of ATM-pSer1981-positive cells (B) in the S-phase population. Data are means, from biological duplicates. Standard errors are not depicted and are <15% of means. Representative images from 14 days are shown below the graphs; right column, ATM activation; left column, H2AX phosphorylation. Scale bar: 10 µm.