Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 4;5:55. doi: 10.1038/s41378-019-0099-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Schematic of the microfluidic chip with 1026 individual microfluidic chambers used for single-cell analysis. Protein quantification was performed by immunoassays with fluorescently barcoded beads, which were co-immobilized with the cells of interest. b Images of the chip and microchambers. (I) Photograph of the chip with the tubing used for the supply of the liquids (blue) as well as the eight control lines (red) used to activate the valves. The permanent magnet that is positioned on top of the chip during the entire experimental procedure is not shown. (II) Micrograph of the chamber array depicting the actuated round valves in grey, as well as the surrounding fluorescent solution (green). The columns between the microchambers (in grey) were used to stabilize the microchannel. (III) A composite fluorescent image of one chamber holding a single cell (blue colour) and three barcoded beads (yellow, red and pink colour) for multiplexed immunoassays. c The protocol used for sensitive proteomic profiling included (I) cell capture, (II) medium exchange, (III) co-capture of beads, (IV) cell lysis and incubation, (V) fluorescent labelling and final imaging. (VI) The immunoassays with the beads required a two-step labelling procedure, first with the biotinylated detection antibodies (det. AB) and subsequently with streptavidin-PE (SAPE)