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. 2019 Nov 4;5:55. doi: 10.1038/s41378-019-0099-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schematic illustration of the immunoassay on the bead surface. Labelling was conducted in two steps with biotinylated antibodies (step 1) and streptavidin-conjugated Phycoerythrin (step 2). (b) Calibration of GAPDH, Gal-3 and Gal-3bp in a 96-well plate format. c Multiplexing with all three targets revealed that there was no cross-reaction between the assays. The digital combinations of the three targets are noted underneath the table, with (“1”) indicating the presence and (“0”) indication the absence of the analyte (d–f). On-chip calibration curves in microchambers with the respective limits of detection (LODs). The blue dotted lines represent the corresponding background signals plus three times the standard deviation. The red lines represent the linear interpolation fit (please note the semi-logarithmic x-axis scaling that results in the nonlinear appearance of the linear interpolation curves)