Skip to main content
. 2019 Oct 4;203(10):2724–2734. doi: 10.4049/jimmunol.1900640

FIGURE 3.

FIGURE 3.

NLRP3 regulates lung tissue repair following N. brasiliensis infection. WT and Nlrp3−/− mice were infected with N. brasiliensis (Nb), and (A) day 7, postinfected BAL alveolar (alv.) Mφ (CD11b−Siglec-F+CD11c+), neutrophil (CD11b+Siglec-F−Ly6G+), and eosinophil (CD11b+Siglec-F+) absolute numbers were measured by flow cytometry. H&E staining was performed on lung sections and imaged followed by (B) quantification of lacunarity (Λ) to assess lung damage on day 7. (C) Representative image insets are shown on top with low magnification scans of entire left lung lobes from individual mice shown below (top panel scale bar, 1000 μm; bottom panel scale bar, 500 μm). (D) Immunofluorescence staining of lung inflammatory foci with SiglecF (red), CD68 (green), and DAPI (blue) (scale bar, 100 μm). (E) Representative day 2 postinfection lung sections and lacunarity (scale bar, 500 μm). Data were pooled (A, B, and E; mean ± SEM) from three individual experiments with three to five mice per group (per experiment). *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA and Tukey–Kramer post hoc test.