FIGURE 4.

NLRP3 regulates Ym1 following infection with N. brasiliensis. Postinfection of WT and Nlrp3^−/− mice with N. brasiliensis (Nb), (A) IL-4 protein levels were measured in lung tissues by ELISA on day 2 postinfection (normalized to tissue weight). (B) Chil3 (Ym1) gene expression was measured by qRT-PCR on day 7 postinfection (log₂ expression relative to Rpl13a). (C) BALF Ym1 was measured by ELISA and (D) intracellular expression was measured by gMFI (fold change over WT control) by alveolar (alv.) Mφs, and neutrophils were quantified by flow cytometry on day 7 postinfection. Data are representative (A; mean ± SEM) or pooled (B–D; mean ± SEM) from three individual experiments with three to five mice per group (per experiment). *p < 0.05, **p < 0.01, one-way ANOVA and Tukey–Kramer post hoc test.