FIGURE 6.

NLRP3 regulates resident Mφ alternative activation by IL-4. WT and Nlrp3^−/− mice were injected with either PBS or IL-4c, and (A) peritoneal CD11b⁺ cells containing F4/80^lo and F4/80^hiTim4⁺ Mφs where analyzed by flow cytometry after 24 h. Frequency and gMFI of intracellular Ym1 and RELM-α were determined in (B) resident F4/80^hiTim4⁺ Mφs and (C) monocyte-derived F4/80^lo Mφs. Data are representative (B, left panel; mean ± SEM) or pooled (B, right panel, and C; mean ± SEM) from three individual experiments with three to five mice per group (per experiment). *p < 0.05, ****p < 0.0001, one-way ANOVA and Tukey–Kramer post hoc test.