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. 2019 Sep 27;18(6):e13037. doi: 10.1111/acel.13037

Figure 6.

Figure 6

Lifelong choline supplementation alters the expression of the Sigma‐1 receptor (σ1R) within microglia. (a‐b) Representative Western blot of σ1R levels. Quantitative analysis of σ1R protein levels reveals a significant reduction with Ch+ (p < .05; n = 5/APP group; n = 4/NonTg group). (c‐d) Quantitative analysis reveals a significant main effect of genotype, where the APP/PS1 mice have a significantly higher intensity of yellow pixels of σ1R/Iba1 colocalization than the NonTg mice (p < .05, n = 6 APP/PS1 CTL, n = 5 APP/PS1 Ch+, n = 6 NonTg CTL, n = 6 NonTg Ch+). Additionally, we find a significant genotype by diet interaction where the APP/PS1 Ch+ mice show a significant reduction in σ1R/Iba1 colocalization than the APP/PS1 CTL mice (p < .001). Photomicrographs depicting the Cornus Ammonis 1 (CA1) of the hippocampus from APP/PS1 and NonTg mice fluorescently stained for the σ1R and Iba1; images taken at 40×; scale bar = 25 µm. Data are presented as box plots. The center line represents the median value, the limits represent the 25th and 75th percentile, and the whiskers represent the minimum and maximum value of the distribution. *p < .05, ***p < .001