RTM significantly improved the reproductivity of aging mice. (a) Cumulative litter size curve showed that RTM (red curve) significantly increased the fertility of the aging mice. Relative age axes of mouse and human were shown below. (b) Numbers of total litters in RTM, MTM, and young groups were significantly more than in the aging group. (c) Numbers of pups per litter (number of total pup/number of litters) were significantly more in RTM, MTM, and young groups than in the aging group. (d) The time from mating to the first birth was significantly shorter in RTM, MTM, and young groups than in the aging group. (e) Percentage of dead pups in RTM, MTM, and young groups were significantly less than in the aging group. *p < 0.05, **p < 0.01, ***p < 0.001 are considered significantly different