Figure 4.

RTM significantly rescued the expression levels of multiple proteins and genes indicative of follicle reserve and oocyte quality. (a) Western blot showed that compared with the aging group, RTM or MTM treatment significantly rescued the protein levels of p‐RPS6 and p‐AMPK close to the young group. (b,c) Quantification of (a). (d) qPCR showed that GDF9 and BMP15 mRNA levels in RTM, MTM, and young groups were significantly higher than in aging group, while mRNA levels of LHR, Cyp19a, and Sirt1 in RTM, MTM, and young groups were significantly lower than in the aging group. *p < 0.05, **p < 0.01, ***p < 0.001 are considered significantly different