Figure 3.

Hyperacetylation of α‐tubulin in old oocytes. (a) Metaphase II oocytes were immunolabeled with acetylated α‐tubulin antibody (green) and counterstained with PI to show chromosomes (red). Representative confocal images of acetylated tubulin in young and old oocytes. (b) Quantification of fluorescence intensity of acetylated tubulin in young (n = 17) and old (n = 19) oocytes. Error bars indicate ± SD. Scale bars: 5 µm. (c) Representative Western blots showing the expression of acetylated tubulin in oocytes from young and old mice. (d) Quantification of Western blot results, with actin as a loading control.* p < .05 versus controls