Figure 3.

Localization of macrophages in the vicinity of adipocytes in the perimysium. (a) Double immunostaining of CD68 (green) and Perilipin 1 (red) to identify macrophages and adipocytes, respectively, in human Y and O skeletal muscle sections. (b) Relative proximity of M2 macrophages (red) and adipocytes (green) in both Y and O muscle sections. (c) Oil Red O staining of lipid droplets (red) in the perimysium. Representative sizes of droplets in O relative to Y skeletal muscle. (d) Double immunostaining of satellite cells (PAX7, green) and M2 macrophages (CD206, red) to visualize their relative localization in human Y, M, and O skeletal muscle sections