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. 2019 Oct 30;12:8905–8914. doi: 10.2147/OTT.S227920

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© 2019 Xu et al.

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miR-186-5p binds directly to HOXA9. (A) The dual luciferase reporter assay validated the direct targeting of miR-186-5p with HOXA9; (B) qRT-PCR verified the expression level of HOXA9 after transfection of miR-186-5p in TE-1 and EC-109 cell lines; (C) qRT-PCR was used to detect the difference in expression of HOXA9 in tumor tissues and adjacent tissues of esophageal carcinoma; (D) qRT-PCR was used to detect the expression level of HOXA9 in esophageal cancer cell lines; (E) There was a significant negative correlation between miR-186-5p and HOXA9 expression in esophageal cancer tissues. Data are mean ± SD, *P<0.05, **P<0.01, ***P<0.001.