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. 2019 Oct 30;12:8905–8914. doi: 10.2147/OTT.S227920

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© 2019 Xu et al.

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miR-186-5p regulates the mechanism of action of HOXA9 to inhibit the development of esophageal cancer. (A) HOXA9 expression levels in eR esophageal cancer TE-1 and EC-109 cell lines co-transfected with miR-186-5p and HOXA9 were detected by qRT-PCR; (B) The CCK-8 assay detects the effect of co-transfection of miR-186-5p and HOXA9 overexpression vectors on proliferation of esophageal cancer cells in TE-1 and EC-109 cell lines; (C) Plate cloning experiments were performed to detect the number of esophageal cancer proliferation-positive cells after co-transfection of miR-186-5p and HOXA9 overexpression vectors in TE-1 and EC-109 cell lines; (D) Transwell assay was used to detect the effect of co-transfection of miR-186-5p and HOXA9 overexpression vectors on the migration ability of esophageal cancer cells in TE-1 and EC-109 cell lines. Data are mean ± SD, *P<0.05, # P<0.05.