Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 30;12:8895–8904. doi: 10.2147/OTT.S222608

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Huang et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Identification of TIMP1 as a potential biomarker for the worse prognosis of patients with CRC. (A) TIMP1 is significantly upregulated in CRC tissues compared with normal colorectal tissues by GEPIA. *significantly different from normal tissues; *p < 0.05. (B) The expression of TIMP1 in the CRC and non-malignant colorectal tissues. Representative immunohistochemistry images were retrieved from the Human Protein Atlas online database. Magnification, x40. (C) Survival analysis of the correlation between TIMP1 expression and overall survival in patients with CRC generated from GEPIA. Log rank tests were employed to determine the statistical significance. (D) Relative expression of TIMP1 in RKO cells after transfection with 100 nmol/L TIMP1 siRNA (siTIMP1) and control siRNA (siControl) for 48 h as assessed by qRT-PCR. Data are shown as mean ± SD. **p < 0.01. (E) Cell proliferation analysis of RKO cells after transfection with 100 nmol/L siTIMP1 and siControl for 48 h. Data are shown as mean ± SD. Two independent experiments were performed. (F) Flow cytometric analysis of Annexin V/PI staining in RKO cells after transfection with 100 nmol/L siTIMP1 and siControl for 48 h. (G) RKO cells exhibited increased cell migration after transfection with 100 nmol/L siTIMP1 and siControl as assessed by the scratching wound-healing assay. Data are shown as mean ± SD. **p < 0.01. Two independent experiments were performed. Unpaired Student’s t-test was performed (D, E, and G).