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. 2019 Oct 15;38(21):e102361. doi: 10.15252/embj.2019102361

Figure EV5. ATX3 prevents excessive 5′‐DNA end resection at sites of DNA damage.

Figure EV5

  1. Representative IF micrographs of U2OS cells showing IR‐induced foci for endogenous RAP80 and γ‐H2AX under indicated siRNA‐depleted conditions 2 h after IR (2 Gy) treatment. Scale bar: 10 μm.
  2. Quantification of (A). Graph represents the average number of RAP80 foci per nucleus (ns P > 0.05, *P < 0.05; unpaired t‐test, n = 2, mean + SEM, at least 100 nuclei per condition and experiment).
  3. Representative IF micrographs in U2OS cells showing IRIF for endogenous RAD51 and γ‐H2AX under indicated siRNA‐depleted conditions 6 h after IR (2 Gy) treatment. EdU staining was used to label S‐phase cells. Scale bar: 10 μm.
  4. Quantification of (C). Graph represents the average number of RAD51 foci per EdU‐positive nucleus (***P < 0.001; unpaired t‐test, n = 2, mean + SEM, at least 100 nuclei per condition and experiment).
  5. Graph representing average number of Rad51 foci per S‐phase (EdU‐positive) nucleus in U2OS∆ATX3 cells as compared with control at different time points (0, 1 and 5 h) after 2 Gy of IR treatment (*P < 0.05, **P < 0.01; unpaired t‐test, n = 3, mean + SEM, at least 100 nuclei were counted per condition and experiment).
  6. Graph representing average number of pRPA foci per S‐phase (EdU‐positive) nucleus in U2OS∆ATX3 cells as compared with control at different time points (0, 0.5, 1 and 5 h) after 2 Gy of IR treatment (*P < 0.05, **P < 0.01; unpaired t‐test, n = 3, mean + SEM, at least 100 nuclei were counted per condition and experiment).